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pcag cas9 ires gfp vector  (Integrated DNA Technologies)


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    Integrated DNA Technologies pcag cas9 ires gfp vector
    Rpl29 methylation shows no effect on global protein synthesis. A, Western blotting showing the absence of Rpl29 in Rpl29−/− NIH 3T3 clones generated by <t>CRISPR-Cas9.</t> B, exogenous Rpl29 or the K5R mutant (untagged) was stably expressed in Rpl29−/− NIH 3T3 cells, and the cell lysates were immunoblotted with Rpl29 antibody and D8T9P, respectively. C, SUnSET assay showing global protein synthesis in the indicated cell lines. As a control for protein synthesis inhibition, NIH 3T3 cells were treated with cycloheximide (CHX, 100 μg/ml) for 12 h before the addition of puromycin. D, [35S]methionine labeling assay showing global protein synthesis in the indicated cell lines. Shown are the mean ± S.D. from three independent experiments. The significance of differences was determined with one-way analysis of variance (ANOVA). *, p < 0.05; ns, not significant. E, polysome profiling showing similar fractions of mRNPs and polysomes in all cell lines.
    Pcag Cas9 Ires Gfp Vector, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 4157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcag cas9 ires gfp vector/product/Integrated DNA Technologies
    Average 99 stars, based on 4157 article reviews
    pcag cas9 ires gfp vector - by Bioz Stars, 2026-02
    99/100 stars

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    1) Product Images from "Identification of Rpl29 as a major substrate of the lysine methyltransferase Set7/9"

    Article Title: Identification of Rpl29 as a major substrate of the lysine methyltransferase Set7/9

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA118.002890

    Rpl29 methylation shows no effect on global protein synthesis. A, Western blotting showing the absence of Rpl29 in Rpl29−/− NIH 3T3 clones generated by CRISPR-Cas9. B, exogenous Rpl29 or the K5R mutant (untagged) was stably expressed in Rpl29−/− NIH 3T3 cells, and the cell lysates were immunoblotted with Rpl29 antibody and D8T9P, respectively. C, SUnSET assay showing global protein synthesis in the indicated cell lines. As a control for protein synthesis inhibition, NIH 3T3 cells were treated with cycloheximide (CHX, 100 μg/ml) for 12 h before the addition of puromycin. D, [35S]methionine labeling assay showing global protein synthesis in the indicated cell lines. Shown are the mean ± S.D. from three independent experiments. The significance of differences was determined with one-way analysis of variance (ANOVA). *, p < 0.05; ns, not significant. E, polysome profiling showing similar fractions of mRNPs and polysomes in all cell lines.
    Figure Legend Snippet: Rpl29 methylation shows no effect on global protein synthesis. A, Western blotting showing the absence of Rpl29 in Rpl29−/− NIH 3T3 clones generated by CRISPR-Cas9. B, exogenous Rpl29 or the K5R mutant (untagged) was stably expressed in Rpl29−/− NIH 3T3 cells, and the cell lysates were immunoblotted with Rpl29 antibody and D8T9P, respectively. C, SUnSET assay showing global protein synthesis in the indicated cell lines. As a control for protein synthesis inhibition, NIH 3T3 cells were treated with cycloheximide (CHX, 100 μg/ml) for 12 h before the addition of puromycin. D, [35S]methionine labeling assay showing global protein synthesis in the indicated cell lines. Shown are the mean ± S.D. from three independent experiments. The significance of differences was determined with one-way analysis of variance (ANOVA). *, p < 0.05; ns, not significant. E, polysome profiling showing similar fractions of mRNPs and polysomes in all cell lines.

    Techniques Used: Methylation, Western Blot, Clone Assay, Generated, CRISPR, Mutagenesis, Stable Transfection, Inhibition, Labeling



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    pcag cas9 ires gfp vector  (Integrated DNA Technologies)
    99
    Integrated DNA Technologies pcag cas9 ires gfp vector
    Rpl29 methylation shows no effect on global protein synthesis. A, Western blotting showing the absence of Rpl29 in Rpl29−/− NIH 3T3 clones generated by <t>CRISPR-Cas9.</t> B, exogenous Rpl29 or the K5R mutant (untagged) was stably expressed in Rpl29−/− NIH 3T3 cells, and the cell lysates were immunoblotted with Rpl29 antibody and D8T9P, respectively. C, SUnSET assay showing global protein synthesis in the indicated cell lines. As a control for protein synthesis inhibition, NIH 3T3 cells were treated with cycloheximide (CHX, 100 μg/ml) for 12 h before the addition of puromycin. D, [35S]methionine labeling assay showing global protein synthesis in the indicated cell lines. Shown are the mean ± S.D. from three independent experiments. The significance of differences was determined with one-way analysis of variance (ANOVA). *, p < 0.05; ns, not significant. E, polysome profiling showing similar fractions of mRNPs and polysomes in all cell lines.
    Pcag Cas9 Ires Gfp Vector, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcag cas9 ires gfp vector/product/Integrated DNA Technologies
    Average 99 stars, based on 1 article reviews
    pcag cas9 ires gfp vector - by Bioz Stars, 2026-02
    99/100 stars
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    Rpl29 methylation shows no effect on global protein synthesis. A, Western blotting showing the absence of Rpl29 in Rpl29−/− NIH 3T3 clones generated by CRISPR-Cas9. B, exogenous Rpl29 or the K5R mutant (untagged) was stably expressed in Rpl29−/− NIH 3T3 cells, and the cell lysates were immunoblotted with Rpl29 antibody and D8T9P, respectively. C, SUnSET assay showing global protein synthesis in the indicated cell lines. As a control for protein synthesis inhibition, NIH 3T3 cells were treated with cycloheximide (CHX, 100 μg/ml) for 12 h before the addition of puromycin. D, [35S]methionine labeling assay showing global protein synthesis in the indicated cell lines. Shown are the mean ± S.D. from three independent experiments. The significance of differences was determined with one-way analysis of variance (ANOVA). *, p < 0.05; ns, not significant. E, polysome profiling showing similar fractions of mRNPs and polysomes in all cell lines.

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of Rpl29 as a major substrate of the lysine methyltransferase Set7/9

    doi: 10.1074/jbc.RA118.002890

    Figure Lengend Snippet: Rpl29 methylation shows no effect on global protein synthesis. A, Western blotting showing the absence of Rpl29 in Rpl29−/− NIH 3T3 clones generated by CRISPR-Cas9. B, exogenous Rpl29 or the K5R mutant (untagged) was stably expressed in Rpl29−/− NIH 3T3 cells, and the cell lysates were immunoblotted with Rpl29 antibody and D8T9P, respectively. C, SUnSET assay showing global protein synthesis in the indicated cell lines. As a control for protein synthesis inhibition, NIH 3T3 cells were treated with cycloheximide (CHX, 100 μg/ml) for 12 h before the addition of puromycin. D, [35S]methionine labeling assay showing global protein synthesis in the indicated cell lines. Shown are the mean ± S.D. from three independent experiments. The significance of differences was determined with one-way analysis of variance (ANOVA). *, p < 0.05; ns, not significant. E, polysome profiling showing similar fractions of mRNPs and polysomes in all cell lines.

    Article Snippet: J1 mESCs or NIH 3T3 cells were transiently co-transfected with the pCAG-Cas9-IRES-GFP vector and two synthesized gBlocks (Integrated DNA Technologies) containing the U6 promoter and Rpl29 targeting sequences in intron 1 and exon 4, respectively ( Fig. S1 A ).

    Techniques: Methylation, Western Blot, Clone Assay, Generated, CRISPR, Mutagenesis, Stable Transfection, Inhibition, Labeling